Head: Dr Phyllis Lam
The Environmental Sequencing Facility provides a range of next-generation sequencing and analysis services using the latest cutting-edge technologies. Our experienced staff offer a wealth of knowledge in this field and understand the complex requirements of modern research environments.
Based within Ocean and Earth Science at the National Oceanography Centre Southampton, the Environmental Sequencing Facility is part of a world-class research institution.
Our services
We are able to provide services covering the whole next generation sequencing workflow, including:
- Advice on experimental design
- Library preparation and quality control
- Sequencing
- Data analysis and interpretation
- Quotes for grant applications
Our capabilities
The majority of our digital sequencing activity is delivered by the Illumina MiSeq sequencer. This versatile instrument carries out a range of applications including:
- Whole genome sequencing of small genomes
- RNA sequencing
- Amplicon sequencing
- ChIP-seq
- Methylation profiling
- Exome sequencing
- Custom target capture
- RADseq
The Illumina MiSeq offers a number of advantages including:
- The fast run time – results can be generated in 3–55 hours, depending on the read length and chemistry used
- Long read length – the MiSeq can generate paired-end reads of up to 300 bp in length, the longest Illumina reads currently available
- Up to 25 million read pairs can be generated per run - a total of 15 Gb data if a read length of 2x300bp is used
Other equipment
- Automated liquid handling capabilities, using the Biomek 4000 liquid handling robot and the Formulatrix Mantis automated liquid dispenser
- Roche LightCycler qPCR instruments
- Covaris M220 for reproducible DNA fragmentation
- Sample tracking using FluidX barcoded tubes
- Bioinformatics capabilities: we run a dedicated analysis server and filestore
- Biomek 4000 for assay development other than NGS
Staff
Dr Phylllis Lam - Associate Professor in Microbial Biogeochemistry within the School of Ocean and Earth Science.
Nicola Pratt - Senior Research Technician, supervises the marine biology technical team and provides technical support in a wide variety of research areas.
Alison Baylay - Post-doctoral research fellow, specialises in developing methods and pipelines for marine metagenomic and transcriptomic studies using the illumina sequencing platform.
Spencer Long - Environment Sequencing Facility Manager.
Contact details
For further details and sequencing enquiries contact
Dr Alison Baylay
[email protected]
023 80596446
456/18
Ocean and Earth Science
National Oceanography Centre Southampton
University of Southampton, Waterfront Campus
European Way
Southampton SO14 3ZH
United Kingdom
Pricing
Please enquire for pricing
