Module overview
Most biological processes involve interactions between macromolecules. The module discusses selected examples and explains techniques used to study molecular interactions.
Linked modules
BIOL2010 OR BIOL2012
Aims and Objectives
Learning Outcomes
Learning Outcomes
Having successfully completed this module you will be able to:
- regulation of kinase activity;
- describe protein ligand interactions;
- read and interpret original literature in molecular recognition.
- describe hydrogen–deuterium exchange in mass spectrometry to study molecular interaction;
- multi-domain proteins, their structure and function;
- describe the structure of RNA and the nature of riboswitches;
- describe the structure of DNA and the nature of interactions with proteins;
- describe GTPases as molecular protein switches;
- present a scientific poster to a lay scientific audience and fellow students;
- describe how peptide motifs are recognised by interacting proteins;
Syllabus
The module discusses ways in which signal transduction events lead to altered gene expression. Cytokines and growth hormones and their receptors are discussed, affecting cell cycle regulation. Protein-peptide recognition will be introduced, and will see how viruses upset cell regulation through interfering peptides. The receptors themselves are often multi-domain proteins. Assembly of multiple domains is a requirement for regulation and efficient binding site presentation. The structure of DNA and the recognition of nucleic acids by interacting proteins such as histones, nucleases, and transcription factors is introduced. Also discussed are the structures of RNA and riboswitches. Methods to study macromolecular interactions are introduced in all sections. The seminal technique of mass spectrometry is covered in detail to explain protein-ligand interactions. Hydrogen–deuterium exchange (HDX) mass spectrometry allows to studying protein-protein interactions. Further, you will learn how to interpret a recent research paper with a focus on methods used to study molecular interactions.
Learning and Teaching
Teaching and learning methods
Lectures and independent study. An exercise to interpret a recent research paper related to the topics of the module in the format of an orally presented poster.
Type | Hours |
---|---|
Guided independent study | 2 |
Independent Study | 94 |
Completion of assessment task | 30 |
Lecture | 24 |
Total study time | 150 |
Assessment
Summative
This is how we’ll formally assess what you have learned in this module.
Method | Percentage contribution |
---|---|
Written assessment | 65% |
Oral presentation | 35% |
Referral
This is how we’ll assess you if you don’t meet the criteria to pass this module.
Method | Percentage contribution |
---|---|
Written assessment | 65% |
Coursework | 35% |