Editing life: genetic engineering and synthetic biology

Learning Outcomes

Learning Outcomes

Having successfully completed this module you will be able to:

• a sound and critical understanding of the molecular biology techniques used to achieve genetic modification
• a critical appreciation of the ethical issues and likely societal impacts that are associated with the different aspects of genetic modification and synthetic biology
• an understanding of the main current research trends in synthetic biology and how they are likely to develop over the next decade
• strategies for acquiring, collating, interpreting, evaluating and presenting complex technical information from cutting-edge research publications
• a broad and critical understanding of the expected technological applications that will stem from the development of synthetic biology
• a basic understanding of adventure and innovation in cross-disciplinary research
• a sound understanding of the different aspects of chemistry that are involved in genetic modification and synthetic biology and how they are used

The philosophy underlying this course is to empower students to take charge of their own learning in the area of synthetic biology. As a consequence the course will make extensive use of directed and peer-assisted self-learning methods.

The module will be delivered in the context of 3 genome engineering research targets:

• genome modification
• assembly of synthetic genomes for minimal genome organisms
• engineering of biochemical pathways for biotechnology applications

The module will cover four broad areas:

Genome editing, modification and synthesis techniques Topics include transformation, Zinc Finger Nucleases, TALENS,

Nucleic acids and Genomes This includes: transcription and translation of DNA; genome architectures (bacterial chromosomes, plasmids); genome processes; basic principles of gene regulation in prokaryotes and eukaryotes; polymerase chain reaction; siRNA.

Genetic circuits Topics include: logic elements from simple gene circuits; gene network energetics; predicting properties of genetic networks.

Societal impacts of genetic engineering. Topics include: ecosystem implications of germ-line genetic modification; unconstrained release of GMOs; enhancing human, animal or plant attributes.

The module will consist of a small number of ‘traditional' lectures, which will be used to deliver some of the key background knowledge in areas such as nucleic acid chemistry, basic biochemistry, molecular biology, gene regulation, screening methods, genetic circuits etc. These lectures will provide the framework for a series of interactive sessions that involve directed self-learning activities centred around the use of primary literature sources. There will be several group-work activities, presentation and discussion sessions as well as workshops. The module will end with a mini-symposium at which they present the results of their final assignment.

Formative

This is how we’ll give you feedback as you are learning. It is not a formal test or exam.

Workshop activities

• Assessment Type: Formative
• Feedback:
• Final Assessment: No
• Group Work: No

Summative

This is how we’ll formally assess what you have learned in this module.

Referral

This is how we’ll assess you if you don’t meet the criteria to pass this module.