Super-resolution microscope

More Information

About the Super-resolution microscope

We have a super-resolution microscope which provides a range of advanced imaging techniques.

Super-resolution microscopy allows us to break the "Diffraction limit", which limits most fluorescence microscopes to around 200 nm resolution. This allows us to visualise molecule localisations and structions that were previously unresolvable. 

Super-resolution microscopy can offer up to 10 times the conventional resolution than a standard microscope. 

We offer both:

  • structured Illumination Microscopy (SIM)
  • stochastic optical reconstruction microscopy (STORM)

Part of: Imaging and microscopy centre

Technical specification

Deltavision OMX Flex multi-modal microscope
Modality and resolution
  • 3D SIM - 100 nm
  • SMLM - 20 nm
  • line-scan Confocal - 200 nm
  • ring-TIRF - 200 nm
  • widefield deconvolution - 200 nm
  • DIC - 250 nm
Objectives
Magnification and numerical aperture
  • 60x, 1.5
  • 60x TRIF, 1.49
Specimen format
  • standard glass slide
  • 35 mm coverslip bottom dish
  • coverslip bottom chamber-slide
Detectors

3 x liquid cooled sCMOS. 1024 x 1024 pixels.

Lasers/filters
Excitation laser λ and emission filters
  • 405 nm - Blue
  • 488 nm  - Green
  • 561 nm  - Red
  • 642 nm  -  Far red
Other features
  • transmitted light - Yes
  • DIC - Yes
  • environmental temperature/gas control - Yes
  • hardware autofocus - Yes
  • simultaneous fluorescence acquisition - Up to 3 channels
  • sequential fluorescence acquisition - Up to 4 channels
  • mark and find - Yes
  • motorised stage - Yes
  • tile scan (stitch multiple FOV together) - No
  • time-lapse - Yes
  • photo-kinetics - Yes
  • 3D imaging - Yes
  • 3D Specimen penetration - SIM: 20 µm  Line-scan: 50 µm 
     



Contact us

Dr Mark Willett

Highfield

Building 85, Highfield Campus, Southampton SO17 1BJ
We’re open Monday to Friday 09:00 to 17:00 UK time.